| 1 |
 Cheltenham Town Ladies
May 15th, 1999 to April 19th, 2016
|
12 |
| 2 |
 Southampton Women's FC
February 2nd, 1997 to November 25th, 2001
|
11 |
| 3 |
 Yeovil Town Ladies
March 26th, 2000 to October 23rd, 2005
|
10 |
| 4 |
 Launton Ladies
September 14th, 1997 to April 30th, 2006
|
7 |
| 5 |
 Portsmouth FC Ladies
February 8th, 1998 to April 15th, 2001
|
7 |
| 6 |
 Denham United Ladies
February 7th, 1999 to December 3rd, 2000
|
7 |
| 7 |
 University of Portsmouth Community Women
September 18th, 2011 to December 8th, 2013
|
7 |
| 8 |
 Bath City Ladies
November 8th, 1998 to October 31st, 2004
|
6 |
| 9 |
 Bristol City Ladies
November 15th, 1998 to May 6th, 2001
|
6 |
| 10 |
 Clevedon Town Ladies
August 26th, 2001 to October 19th, 2003
|
6 |
| 11 |
Reading Ladies
March 2nd, 2003 to February 13th, 2005
|
6 |
| 12 |
 Southampton Saints Ladies
September 11th, 2011 to September 15th, 2013
|
6 |
| 13 |
 Exeter City Ladies
December 11th, 2011 to October 13th, 2013
|
6 |
| 14 |
 Poole Town Ladies
November 11th, 2012 to March 27th, 2022
|
6 |
| 15 |
 Shanklin Ladies
October 20th, 2013 to May 1st, 2016
|
6 |
| 16 |
 Larkhall Athletic Ladies
December 7th, 2014 to November 4th, 2018
|
6 |
| 17 |
 Keynsham Town Ladies
October 13th, 2022 to August 28th, 2024
|
6 |
| 18 |
 Reading Royals Ladies
October 10th, 1993 to September 10th, 1995
|
5 |
| 19 |
Reading Ladies
May 9th, 1995 to September 23rd, 2001
|
5 |
| 20 |
 Southampton Saints Ladies
September 7th, 1997 to February 19th, 2008
|
5 |
| 21 |
Exeter City Ladies
February 23rd, 2003 to August 15th, 2009
|
5 |
| 22 |
 AFC TeamBath Ladies
March 7th, 2009 to September 4th, 2014
|
5 |
| 23 |
Southampton Saints Ladies
September 14th, 2014 to September 13th, 2015
|
5 |
| 24 |
 Oxford City Ladies
March 16th, 2005 to January 15th, 2006
|
4 |
| 25 |
Clevedon Town Ladies
January 29th, 2006 to February 18th, 2007
|
4 |
| 26 |
Reading Royals Ladies
October 19th, 2008 to February 28th, 2010
|
4 |
| 27 |
 Wootton Bassett Town Ladies
January 9th, 2011 to February 16th, 2025
|
4 |
| 28 |
 St. Nicholas Ladies
August 24th, 2014 to April 24th, 2016
|
4 |
| 29 |
 Chichester City Ladies
September 7th, 2014 to March 13th, 2016
|
4 |
| 30 |
 Chichester and Selsey Ladies
October 30th, 2022 to March 24th, 2024
|
4 |
| 31 |
 Southampton Women's FC
September 17th, 2023 to January 12th, 2025
|
4 |
| 32 |
 Bridgwater United Women
September 1st, 2024 to March 29th, 2026
|
4 |
| 33 |
 Worthing Women
September 22nd, 2024 to March 22nd, 2026
|
4 |
| 34 |
 Salisbury City Ladies
December 7th, 1997 to October 19th, 2025
|
3 |
| 35 |
Oxford City Ladies
December 17th, 2006 to March 20th, 2009
|
3 |
| 36 |
 Winscombe Ladies
September 26th, 2009 to March 28th, 2010
|
3 |
| 37 |
 Gloucester City Ladies
August 29th, 2010 to September 25th, 2011
|
3 |
| 38 |
 Melksham Town Ladies
March 24th, 2013 to December 17th, 2023
|
3 |
| 39 |
 Torquay United Ladies
November 2nd, 2014 to May 5th, 2024
|
3 |
| 40 |
Keynsham Town Ladies
September 7th, 2025 to March 8th, 2026
|
3 |
| 41 |
Aylesbury United Ladies
May 3rd, 1995 to May 4th, 1995
|
2 |
| 42 |
Portsmouth Ladies Reserves
May 7th, 1995 to May 8th, 1995
|
2 |
| 43 |
 Slough Ladies
May 13th, 1995 to May 14th, 1995
|
2 |
| 44 |
Eastleigh Ladies
September 17th, 1995 to November 19th, 1995
|
2 |
| 45 |
 Oxford University Women
October 22nd, 1995 to January 21st, 1996
|
2 |
| 46 |
 Havant Town Ladies
September 15th, 1996 to October 27th, 1996
|
2 |
| 47 |
Havant Town Ladies
August 31st, 1997 to November 16th, 1997
|
2 |
| 48 |
 Reading Royals Ladies
September 2nd, 2001 to November 21st, 2001
|
2 |
| 49 |
Portsmouth FC Ladies
September 30th, 2001 to February 17th, 2002
|
2 |
| 50 |
Clevedon Town Ladies
September 5th, 2004 to November 14th, 2004
|
2 |
| 51 |
 AFC Bournemouth Ladies
October 1st, 2006 to February 25th, 2007
|
2 |
| 52 |
Yeovil Town Ladies
November 11th, 2007 to January 26th, 2008
|
2 |
| 53 |
Oxford City Ladies
November 21st, 2010 to January 16th, 2011
|
2 |
| 54 |
 Havant and Waterlooville Ladies
March 18th, 2012 to April 1st, 2012
|
2 |
| 55 |
Gloucester City Ladies
September 11th, 2012 to February 24th, 2013
|
2 |
| 56 |
Chichester City Ladies
December 9th, 2012 to March 9th, 2014
|
2 |
| 57 |
Exeter City Ladies
September 20th, 2015 to March 27th, 2016
|
2 |
| 58 |
 Cheltenham Town Ladies
April 27th, 2019 to August 25th, 2021
|
2 |
| 59 |
Larkhall Athletic Women
February 24th, 2022 to August 28th, 2022
|
2 |
| 60 |
 AFC Bournemouth Women
September 18th, 2022 to February 5th, 2023
|
2 |
| 61 |
 AFC St. Austell Ladies
January 15th, 2023 to March 5th, 2023
|
2 |
| 62 |
 Purton FC Women
April 14th, 2024 to April 13th, 2025
|
2 |